15-hydroxyeicosatetraenoic acid stimulates migration of human retinal microvessel endothelium in vitro and neovascularization in vivo

J. E. Graeber; B. M. Glaser; B. N.Y. Setty; J. A. Jerdan; R. W. Walenga; M. J. Stuart

doi:10.1016/0090-6980(90)90026-R

15-hydroxyeicosatetraenoic acid stimulates migration of human retinal microvessel endothelium in vitro and neovascularization in vivo

J. E. Graeber
, B. M. Glaser
, B. N.Y. Setty
, J. A. Jerdan
, R. W. Walenga
, M. J. Stuart

Johns Hopkins University
Temple University

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

We evaluated 15-hydroxyeicosatetraenoic acid (15-HETE), a major arachidonic acid product of vascular endothelium and leukocytes, for its effect on neovascularization. In a modified Boyden chamber assay, 15-HETE (10^-7 M) sitmulated human retinal microvessel endothelial cell migration by 42 ± 10% (mean ± S.E.M., p<0.01). 12-HETE, a major arachidonic acid metabolite of platelets, had no such effect. Further studies in the rabbit corneal pocket assay revealed that 15-HETE stimulated neovascularization in vivo. Concentrations at which the in vivo effects were observed are within the range generated by several cell types and are achievable in human serum. 15-HETE stimulation of human endothelial cell migration in vitro and neovascularization in vivo suggests that it may play a role in vasoproliferative disorders.

Original language	English
Pages (from-to)	665-673
Number of pages	9
Journal	Prostaglandins
Volume	39
Issue number	6
DOIs	https://doi.org/10.1016/0090-6980(90)90026-R
State	Published - Jun 1990
Externally published	Yes

Access to Document

10.1016/0090-6980(90)90026-R

Cite this

@article{f077119b93cb44e885b488bc7c852b4b,

title = "15-hydroxyeicosatetraenoic acid stimulates migration of human retinal microvessel endothelium in vitro and neovascularization in vivo",

abstract = "We evaluated 15-hydroxyeicosatetraenoic acid (15-HETE), a major arachidonic acid product of vascular endothelium and leukocytes, for its effect on neovascularization. In a modified Boyden chamber assay, 15-HETE (10-7 M) sitmulated human retinal microvessel endothelial cell migration by 42 ± 10\% (mean ± S.E.M., p<0.01). 12-HETE, a major arachidonic acid metabolite of platelets, had no such effect. Further studies in the rabbit corneal pocket assay revealed that 15-HETE stimulated neovascularization in vivo. Concentrations at which the in vivo effects were observed are within the range generated by several cell types and are achievable in human serum. 15-HETE stimulation of human endothelial cell migration in vitro and neovascularization in vivo suggests that it may play a role in vasoproliferative disorders.",

author = "Graeber, \{J. E.\} and Glaser, \{B. M.\} and Setty, \{B. N.Y.\} and Jerdan, \{J. A.\} and Walenga, \{R. W.\} and Stuart, \{M. J.\}",

year = "1990",

month = jun,

doi = "10.1016/0090-6980(90)90026-R",

language = "English",

volume = "39",

pages = "665--673",

journal = "Prostaglandins",

issn = "0090-6980",

publisher = "Elsevier B.V.",

number = "6",

}

TY - JOUR

T1 - 15-hydroxyeicosatetraenoic acid stimulates migration of human retinal microvessel endothelium in vitro and neovascularization in vivo

AU - Graeber, J. E.

AU - Glaser, B. M.

AU - Setty, B. N.Y.

AU - Jerdan, J. A.

AU - Walenga, R. W.

AU - Stuart, M. J.

PY - 1990/6

Y1 - 1990/6

N2 - We evaluated 15-hydroxyeicosatetraenoic acid (15-HETE), a major arachidonic acid product of vascular endothelium and leukocytes, for its effect on neovascularization. In a modified Boyden chamber assay, 15-HETE (10-7 M) sitmulated human retinal microvessel endothelial cell migration by 42 ± 10% (mean ± S.E.M., p<0.01). 12-HETE, a major arachidonic acid metabolite of platelets, had no such effect. Further studies in the rabbit corneal pocket assay revealed that 15-HETE stimulated neovascularization in vivo. Concentrations at which the in vivo effects were observed are within the range generated by several cell types and are achievable in human serum. 15-HETE stimulation of human endothelial cell migration in vitro and neovascularization in vivo suggests that it may play a role in vasoproliferative disorders.

AB - We evaluated 15-hydroxyeicosatetraenoic acid (15-HETE), a major arachidonic acid product of vascular endothelium and leukocytes, for its effect on neovascularization. In a modified Boyden chamber assay, 15-HETE (10-7 M) sitmulated human retinal microvessel endothelial cell migration by 42 ± 10% (mean ± S.E.M., p<0.01). 12-HETE, a major arachidonic acid metabolite of platelets, had no such effect. Further studies in the rabbit corneal pocket assay revealed that 15-HETE stimulated neovascularization in vivo. Concentrations at which the in vivo effects were observed are within the range generated by several cell types and are achievable in human serum. 15-HETE stimulation of human endothelial cell migration in vitro and neovascularization in vivo suggests that it may play a role in vasoproliferative disorders.

UR - https://www.scopus.com/pages/publications/0025314874

U2 - 10.1016/0090-6980(90)90026-R

DO - 10.1016/0090-6980(90)90026-R

M3 - Article

C2 - 1695384

AN - SCOPUS:0025314874

SN - 0090-6980

VL - 39

SP - 665

EP - 673

JO - Prostaglandins

JF - Prostaglandins

IS - 6

ER -

15-hydroxyeicosatetraenoic acid stimulates migration of human retinal microvessel endothelium in vitro and neovascularization in vivo

Abstract

Access to Document

Other files and links

Fingerprint

Cite this