@inbook{203fd3368936463698b27673c9116d7f,
title = "3D Hydrogel Cultures for High-Throughput Drug Discovery",
abstract = "Our increased understanding of how a cell{\textquoteright}s microenvironment influences its behavior has fueled an interest in three-dimensional (3D) cell cultures for drug discovery. Particularly, scaffold-based 3D cultures are expected to recapitulate in vivo tissue stiffness and extracellular matrix composition more accurately than standard two-dimensional (2D) monolayer cultures. Here we present a 3D hydrogel cell culture setup suitable for automated screening with standard high-throughput screening (HTS) liquid handling equipment commonly found in a drug discovery laboratory. Further, we describe the steps required to validate the assay system for compound screening.",
keywords = "Cell Culture Techniques/methods, Drug Discovery/methods, Extracellular Matrix, High-Throughput Screening Assays/methods, Hydrogels",
author = "Karen Sperle and Pochan, \{Darrin J.\} and Langhans, \{Sigrid A.\}",
note = "{\textcopyright} 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-2914-7\_22",
language = "English",
volume = "2614",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Humana Press Inc.",
pages = "369--381",
booktitle = "Methods in Molecular Biology",
}